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In Vitro Transcription Pcr Template

In vitro transcription pcr template - Generally, you should use an annealing temperature about 5°c below the t m of your primers. The optimal annealing temperature (t a opt) for a given primer pair on a particular target can be calculated as follows: The annealing temperature (t a) chosen for pcr relies directly on length and composition of the primers.

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The annealing temperature (t a) chosen for pcr relies directly on length and composition of the primers. Generally, you should use an annealing temperature about 5°c below the t m of your primers. The optimal annealing temperature (t a opt) for a given primer pair on a particular target can be calculated as follows: